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Cloning, expression, and purification of the His(6)-tagged thermostable beta-galactosidase from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme. Typhoid cholecystitis and gallbladder carcinoma after interval of 67 years. HBc antibody-negative sera, collected before the onset of the acute phase in recovering patients, were used as controls. In the sapwood-heartwood transition zone, flavanol accumulation was correlated with the transcription levels cheap viagra of the chalcone synthase (CHS) and flavanone 3-hydroxylase (F3H) genes.
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